This study aims to show the guideline to select optimal anti-vibration gloves for specific power tools to prevent hazardous vibration to human body. It is most desirable for the correct evaluation of handtransmitted vibration form the power tool handle to measure the acceleration between the handle surface and the hand palm as recommended in ISO 5349-1. First, the accurate acceleration measurement device was developed of which the thickness and weight were less than 6 mm and 12 g respectively so that it can be placed between the handle and the palm without any inconvenience during the measurement. Finally, using the device we estimated anti-vibration glove performances considering the frequency characteristics of generated vibration by the power tool.