Possible anti-inflammatory effect of carvacrol was evaluated by in vitro cyclooxygenase-2 (COX-2) assay. Carvacrol inhibited production of prostaglandin $E_2$ catalysed by COX-2 with an $IC_{50}$ value of $0.8{mu}M$ what is practically the same concentration as the $IC_{50}$ obtained for the standard inhibitors indomethacin and NS-398 with values of $0.7{mu}M$ and $0.8{mu}M$, respectively. The COX-I was inhibited approximately at the same rate ($IC_{50}$ of $0.7{mu}M$ for carvacrol), which suggests non-selective inhibition of both enzyme isoforms. The results of the study demonstrate possible anti-inflammatory potential of this compound due to the inhibition of inducible COX-2 isoform.