40-day-old mixed male puppy was presented with a 10-day history of tenesmus and extensive ulcerative lesion of perineum. At physical examination, the dog was voiding mainly from an orifice in the perineal fistula. On fistulogram, a urethroperineal fistula was diagnosed. The dog was treated by wet dressing for wound management and antibiotics for 4weeks. Subsequently, the patient improved and no complication or recurrence was presented.