We have investigated biosorption kinetics and equilibrium of $Pb^2+$ and $Cr^2+$ using waste sludge, and separation efficiency of waste sludge by dissolved air flotation was evaluated in the various A/S ratio. The biosorption capacity and contact time were shown as a simulation of biosorption equilibrium and kinetics models. Biosorption equilibrium of the $Pb^2+$ and $Cr^2+$ onto the waste sludge could be fitted by the Langmuir, Freundlich, Redlich-Peterson, and Koble-Corrigan equation. The kinetics could be fitted by a pseudo-second-order rate equation more than a pseudo-first-order rate equation. The separation efficiency of waste sludge using DAF was kept above 90%.