Naringenin is a bioactive flavanone containing antioxidative, anti-inflammatory, and anticarcinogenic properties. The inhibitory effects on hyaluronidase of naringenin and its novel derivatives were evaluated. Among these flavonoids at $200{mu}M$ concentration, 7-O-butyl naringenin had the highest inhibitory effect on hyaluronidase with 44.84%. In addition, For naringenin at concentrations of 0, 150, and $190{mu}M$, the apparent Michaelis constants ($_{app}K_m$) were calculated to be $0.60{pm}0.02$, $0.43{pm}0.02$, and $0.41{pm}0.01;mg/mL$ of substrate, respectively; for 7-O-butyl naringenin at 0, 20, and $30{mu}M$ concentrations, those were $0.44{pm}0.03$ and $0.27{pm}0.03;mg/mL$, respectively. The $V_{max}$ values at 150 and $190{mu}M$ naringenin were $0.59{pm}0.02$ and $0.56{pm}0.01;mg/mL/min$, respectively; and those at 20 and $30{mu}M$ 7-O-butyl naringenin were $0.50{pm}0.02$ and $0.33{pm}0.02;mg/mL/min$, respectively. However, the slopes of each inhibitory reaction were not significantly different. Therefore, naringenin and 7-O-butyl naringenin were shown to be uncompetitive inhibitors. These results demonstrate the potential use of 7-O-butyl naringenin as an anti-inflammatory substance.