Six sigma methode was applied for optimization of flow field design of a proton exchange membrane fuel cell (PEMFC). The optimization between number of channel and channel/rib width was suggested in this paper with six sigma method. With the help of six sigma design of experiment (DOE) the number of experiments may be reduced dramatically. The fuel cell channel design optimization with results of these experiments with a 100 $cm^2$ serpentine flow field indicates a optimization data for a given constant operating conditions.