The effects of benomyl treatment on ginsenoside and arbuscular mycorrhizal (AM) symbiosis in the roots of Panax ginseng that were collected from two sites in Korea were investigated. The ginseng roots that were treated with benomyl showed different species compositions of AM fungi colonizing the ginseng roots, compared to untreated roots. In the analysis of ginsenoside, Rc was significantly higher in benomyl untreated roots than in benomyl treated roots. The results suggest that AM fungal species composition and ginsenosides in ginseng root could be influenced by the benomyl treatment.