Objectives : The purpose of this study was to investigate the effect of chuna treatment(manipulation) on lumbar sprain caused by traffic accident in early stage, by analysis of the Heart Rate Variability(HRV) and Visual Analogue Scale(VAS). Methods : This study carried out on 20 patients who have received hospital treatment in Daejeon Univ. cheonan Oriental Hospital. Non-chuna group got acupuncture-therapy, herbal medication, physical therapy and Chuna group got all the therapies and chuna treatment. We measured HRV and VAS on 2nd, 4th day. Results : After being treated by our methods, Chuna group showed the inclination to balance between the sympathetic and parasympathetic nerve. In chuna group as compared with non-chuna group, an autonomic nerve activity and stress resistance showed the inclination to increase(p<0.05). And chuna treatment reduced the rate of stress increase. Conclusions : The results suggest that chuna treatment help traffic accident patients in early stage to reduce pain. Refer to autonomic nerve system, chuna treatment seem to do positive effect but Further long term study in a large scale is needed.