Repetitive movement, inadequate working posture, overuse of force, physical contact with sharp edge, vibration and temperature were well known risk factors of WMSDs (Work-related Musculoskeletal Disorders). Many researchers have investigated the relationship between inadequate working postures and incidences of WMSDs of the upper extremities, whereas relatively few researchers have tried to evaluate workload associated with the lower-body postures. The effects of lower-limb postures including various knee flexion angles on the subjective discomfort, heart rate and EMG of lower-limb muscles were investigated in this study. Thirty graduate students were asked to maintain thirteen different body postures, and heart rate and EMG data of five muscle groups (electro spine, biceps femoris, vastus medialis, gastrocnemius and tibialis anterior) from each posture were collected during fifteen minutes sustaining tasks. All participants were also asked to report their discomfort ratings of body parts. Results showed that high subjective discomfort ratings and heart rates were reported at the postures of knee angles of $60^{circ}$ and $90^{circ}$, whereas low discomfort ratings were founded at the postures of chair heights with 20cm, 40cm, and sitting with crossed legged. The change of median frequency for each muscle group during fifteen minutes tasks was investigated for each body posture to evaluate the relationship between muscle fatigue and body posture. It was found that the trends of changes of median frequency were different based on muscle group as well as lower-limb body posture from this study.