기존의 번데기동충하초의 자실체 생산은 자연계에서 채집된 버섯의 조직을 분리하거나 다중의 포자를 이용하는 방법이 대표적이었으나 본 연구를 수행한 결과, 단포자를 선발한 후 교배조합에 의한 F1균주를 이용한 배양이 안정적임을 확인 할 수 있었다. 단포자의 특성으로는 자실체의 형성이 양호하며 균주의 안정성이 우수한 균주를 선발하고 조합형성능이 우수한 균주를 선발하여야 한다. 본 연구를 통하여 06S05의 단포자를 획득하였으며 06S05${ imes}$06S01, 06S05${ imes}$06S33균주를 선발하였으며 06S05${ imes}$06S33균주를 예당 3호로 명명하여 국립종 자원에 품종보호 등록되었다.
Single ascospores was isolated in Yesan-gun, Chungnam in 2006 with Cordyceps militaris. This study was carried out to select the superior isolates which produce more fruiting body and contain more cordycepin contents. Five single ascospore isolates were cultured on brown rice pupae medium and they formed fruiting bodies. To select better isolates, each isolates was hybridized and then the characteristics and cordycepin contents were investigated. After biological efficiency and codycepin contents were examined, finally C0601S05 was selected. Top-cross test of isolate C0601S05 was carried with 29 single ascospores. From this test, isolate C0601S05${ imes}$C0601S33 and isolate C0601S05${ imes}$C0601S43 containing highest cordycepin contents were selected. Codycepin contents of C0601S05 was 246mg% and those of C0601S05${ imes}$C0601S33 and C0601S05${ imes}$C0601S43 were 325mg% and 280mg%, respectively. C0601S05${ imes}$C0601S33 was registered as Yedang 3 at the Korea seed & variety service.
