Background: The purpose of this study was to investigate the comparison between the walking speed and muscle tone of the lower extremity in male and female. Method: The experimental group was that 22 female and 24 male that participated this study. Subjects were measured walking speed and muscle tone. Muscle tone measured using myotonometer. Measured muscle were rectus femoris, vastus medialis, vastus lateralis, biceps femoris, semitendinousus, tibialis anterior, gastrocnemius. Result: The result of this study were that the walking speed were significantly significant(p<.01) in male and female. Muscle tone different in male and female that vastus medialis, vastus lateralis, tibialis anterior and gastrocnemius significantly significant(p<.05). Conclusion: This study showed that difference muscle tone and walking speed in male and female. This indicates that there is an interaction that plays a crucial roles in the walking speed and muscle tone.