The objective of this study was to analyze the factors regarding work-related musculoskeletal disorders (MSDs) in fruit-growers and offer the fundamental data for work improvement. The 587 fruit-growers (409 males and 178 females) working with 5 kinds of fruit: apple, pear, peach, grape, and mandarin participated in a questionnaire survey regarding MSDs and health condition in 2004 and 2005. The results are as follows: 1. Smoking rate was highest in apple growers. 2. Drinking rates were highest in male peach growers and in female grape growers. 3. Regular exercise rates were higher in pear and mandarin growers. 4. Physical and mental fatigue was higher in females than in males in most cases. 5. The prevalence rate of medically diagnosed diseases was highest for osteoarthritis (16.4%), herniated nucleus pulposus (HNP, 13.5%), and chronic gastritis/gastric ulcer (10.5%). As well as, the prevalence of dermatosis was higher in pear and mandarin growers. 6. The prevalence rate of musculoskeletal symptoms among the various pain areas was highest for lower back, shoulders, and knees. The prevalence rate was also high for lower back, shoulder, and knee pain in apple and grape growers. 7. The significant indexes used determining the musculoskeletal symptoms were BMI, working period, and regular exercise. These results can be practically used for work improvement for the fruit-growers to prevent MSDs.