A stereoscopic micro-PTV (particle tracking velocimetry) technique based on 2-frame PTV using match probability was developed. This method measures not only a two-dimensional velocity field but also the out-of-plane velocity component in micro fluid flow. A validation study of SMPTV by using a simulated flow model and a real micro jet flow was performed to verify the accuracy and feasibility of the method. The effects of different kinds of microscopes and the out-of-focus effect were also investigated. All test results were compared with the SMPIV method in order to evaluate the performance. The results showed that the SMPTV method using a CMO type microscope accurately measured the micro flow with the lowest bias error and higher spatial resolution than the SMPIV method.