Temperature variation happens in micro prism roll mold processing system during machining the prism pattern roll mold using manufacturing optical films of LCD (liquid crystal display). This temperature variation induces pitch errors of the prism patterns. The temperature variation displaces the positions of the diamond cutting tool on the roll which was coated by the copper. In order to prevent the pitch errors, the stabilizing the temperature of machining environment is needed. Therefore, the researching on the temperature variation of the ultra-precision roll mold processing system on the machining of micro prism rot 1 mold is needed. In this paper, the temperature variation of micro prism roll mold processing system is researched, the influence is analyzed, and the study for reducing the pitch errors carried out.