This study was carried out to investigate the general characteristics, such as volume, pH, sperm motility and sperm concentration of the semen collected from Beagle dogs (age $24{sim}48$ months, weight $10{sim}15;kg$) by using the method of digital manipulation of the penis, and the effect of preservation temperature and time on motility of fresh semen. Multiple ejaculates were collected from 4 male Beagles. The average volume, pH, motility and sperm concentration of the second fraction (contained with small volume of the third fraction) per ejaculation were $2.94{pm}0.24(SD);ml$, $6.43{pm}0.42(SD)$, $97.04{pm}3.50(SD)%$ and $1.67{pm}0.23(SD){ imes}10^8;cells/ml$, respectively. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the first fraction from the ejaculate were $1.24{pm}0.20(SD);ml$, $6.03{pm}0.26(SD)$, $1l.30{pm}4.02(SD)%$ and $7.25{pm}1.02(SD){ imes}10^5;cells/ml$. Those of second fraction were $2.52{pm}0.32(SD);ml$, $6.32{pm}0.31(SD)$, $96.25{pm}3.52(SD)%$ and $2.35{pm}0.35(SD){ imes}10^8;cells/ml$. Those of third fraction were $2.71{pm}0.27;(SD);ml$, $6.52{pm}0.20(SD)$, $95.65{pm}2.78(SD)%$ and $5.72{pm}0.29(SD){ imes}10^7;cells/ml$. Motility of semen was higher at $17^{circ}C$ preservation temperature than $5^{circ}C$ or $36^{circ}C$ during preservation period. When preservation temperature was $17^{circ}C$, motility was $96.54{pm}2.05(SD)%$ at 1 h, $90.20{pm}3.90(SD)%$ at 6 h, $89.05{pm}2.01(SD)%$ at 12 h, $78.21{pm}3.50(SD)%$ at 18 h, $45.24{pm}6.25;(SD)%$ at 24 h and $30.75{pm}17.24(SD)%$ at 30 h, respectively.