This study was conducted to evaluate the inflammation mechanisms of tumor necrosis factor-$alpha$ (TNF-$alpha$), interleukin-4 (IL-4), and IL-$1{eta}$-induced stimulation of A549 human epithelial cells. In the present study, A549 cells were stimulated with TNF-$alpha$, IL-4 and IL-$1{eta}$ to induce expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. The effects of TNF-$alpha$, IL-4 and IL-$1{eta}$ on gene expression profiles in A549 cells were evaluated by oligonucleotide microarray and Real time RT-PCR. The gene expression profiles for the A549 cells varied depending on the cytokines. Also, the results of the microarray and Real time RT-PCR revealed that inflammatory-related genes were up-regulated in cytokine stimulated A549 cells. Cytokines can affect inflammation in A549 cells. A microarray-based genomic survey is a high-throughput approach that enables evaluation of gene expression in cytokine stimulated cell lines.