Objectives The purpose of this study is to investigate the effect of Kakamsodokum (KKSDU) on atopic dermatitis in an in-vitro experiment using an NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the condition in humans. Methods We evaluated $IL-1{eta}$, IL-6, $TNF-{alpha}$, $TGF-{eta}$, IL-10 mRNA, CD4+/$IFN-{gamma}+$, and CD4+CD25+foxp3+ in B and T cells of NC/Nga atopic dermatitis mouse by real-time PCR and intracellular staining in vitro. Results KKSDU medicines supressed $IL-1{eta}$, IL-6, $TNF-{alpha}$, $TGF-{eta}$ mRNA and increased IL-10 mRNA in B cells. CD4+/$IFN-{gamma}+$ and CD4+CD25+foxp3+ in T cells were increased by KKSDU. Conclusions KKSDU on atopic dermatitis might be very effective to the atopic dermatitis treatment.