This experimental study was designed to investigate the inhibitory effects of Sojihwangamibang on hyperlipidemia in rats induced by high cholesterol diet diet. Sprague- Dawley rats were divided into normal group, control group, SJB treated group. Obese rats were induced by high cholesterol diet treatment for 6 weeks including a oral administration of SJB for 4 weeks. In SJB group, serum total cholesterol, LDL cholesterol, triglyceride and glucose were significantly decreased, and HDL cholesterol was significantly increased compared with untreated control group. In SJB group, HMG-CoA and ACAT concentration of hepatic homogenate were significantly decreased compared with untreated control group. These results provide experimental evidence that SJB, applied currently in the clinical practice, appears to be effective for down-regulating risk factors of hyperlipidemia, and thus may be used as an objective information for the development of therapeutic agents.