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Development of a Virus Elution and Concentration Procedure for Detecting Norovirus in Cabbage and Lettuce
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  • Development of a Virus Elution and Concentration Procedure for Detecting Norovirus in Cabbage and Lettuce
  • Development of a Virus Elution and Concentration Procedure for Detecting Norovirus in Cabbage and Lettuce
저자명
Moon. Aerie,Hwang. In-Gyun,Choi. Weon-Sang
간행물명
Food science and biotechnology
권/호정보
2009년|18권 2호|pp.407-412 (6 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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In this study, a rapid and efficient concentrating procedure that can be used for detecting viruses in vegetables was developed. The Sabin strain of poliovirus type 1 was used to evaluate the efficiency of virus recovery. The procedure included: (a) elution with 0.25 M threonine-0.3 M NaCl pH 9.5; (b) polyethylene glycol (PEG) 8000 precipitation; (c) chloroform extraction; (d) 2$^{nd}$ PEG precipitation; (f) RNA extraction; (g) reverse transcription-polymerase chain reaction (RT-PCR) combined with semi-nested PCR. The overall recoveries by elution/concentration were 29.0% from cabbage and 13.7% from lettuce. The whole procedure usually takes 18 hr. The overall detection sensitivity was 100 RT-PCR units of genogroup II norovirus (GII NoV)/25 g cabbage and 100 RT-PCR units of GII NoV/10 g lettuce. The virus detecting method developed in this study should facilitate the detection of low levels of NoV in cabbage and lettuce.