Purpose: The purpose of this study was to investigate the relationships between job stress and work-related musculo-skeletal symptoms among nurses working in general hospitals. Method: A descriptive correlational research design was utilized. The participants were 438 nurses currently working in 4 general hospitals. Data were collected from Jan 2007 to Feb 2007, with Korean Occupational Stress Scale(KOSS)-basic form, KOSHA code H-30 and a questionnaire for general characteristics. Descriptive statistics, t-test, ANOVA, multiple logistic regression analyses were utilized. Result: Most participants(90.4%) complained of work-related musculo-skeletal symptoms. However, symptomatic nurses satisfying NIOSH screening criteria(symptomatic nurses) were 66.9%(293 persons). The most complained body part was back(39.5%). shoulder(37.7%), leg and foot(36.5%), wrist and hand(21.7%), neck(18.7%), and arm(9.8%). The mean of total score of occupational stress was 51.11. In multiple logistic regression analyses, age, working posture and total score of occupational stress showed significant associations with back symptoms. Exercise and total score of occupational stress were significantly related with shoulder symptoms. Hours of daily house keeping and working posture were related with leg and foot symptoms. Marital status and working posture were related with wrist and hand symptoms. Working posture only showed significant relations with neck symptom. Marital status, hours of daily house keeping, shift work, working posture, and total score of occupational stress were related with the symptoms in the arm. In conclusion, job stress of nurses might be related with work-related musculo-skeletal symptoms.