Road construction has been worked on by focusing on cost effectiveness such as the reduction of construction costs without understanding the natural environment and ecosystems. In addition, since ecosystems have been destroyed and the habitats of animals and plants have been isolated, wild animals have sought other habitats or have moved into isolated habitats. Thus, issues such as roadkills or the extermination of species are increasingly occurring. Based on this background, the development and application of a general assessment method need to be researched in order to verify whether or not spaces or habitats where animals can be inhabited and their species can be maintained exist. The purpose of this study is to develop an ecosystem map where a conservation value evaluation method considering the functions of an ecosystem where animals live as well as the naturalness of ecosystems is used and can be made into a drawing. The items applied to evaluate the habitat conservation values were naturalness, rarity, and functionality, and sub-items for evaluation were created. GIS as well as the evaluation items were used to create a digital map about the level of importance based on the evaluation criteria. The created digital map showed that it was not limited to the adjustment or distribution of the fauna and flora but was applicable for a general ecosystem evaluation method for the conservation of habitats. It is expected that the isolation of habitats would be able to be minimized if the digital map is used for road construction projects. The digital map is deemed to be able to be used for the construction of environmentally friendly roads, which will minimize the destruction of ecosystems and the isolation of habitats for creatures.