To determine the anti-complement activity of natural triterpenes, chromatographic separation of the EtOAc-soluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of three steroids and five triterpenoids. They were identified as ergosterol peroxide (1), ergosterol (2), genoderic acid Sz (3), stella sterol (4), ganoderic aic C1 (5), ganoderic acid A (6), methyl ganoderate A (7), and lucidenic acid A (8) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their anti-complement activity against the classical pathway of the complement system. Compounds 2 and 3 showed potent anti-complement activity with $IC_{50}$ values of 52.0 and $44.6;{mu}M$, respectively. Compound 1 exhibited significant inhibitory activity with an $IC_{50}$ value of $126.8;{mu}M$, whereas compounds 4- 8 were inactive. Our findings suggested that in addition to the ketone group at C-3, the ${Delta}^{7(8)}$, ${Delta}^{9(11)}$-lanostadiene type triterpene also plays an important role in inhibiting the hemolytic activity of human serum against erythrocytes.