In this work, the evaporation phenomena of 20-45 picoliter water droplet (i.e. 50-65 ${mu}m$ diameter) on heated substrates with different thermal conductivity are studied experimentally. The effect of thermal conductivity of substrates and inter-distance between jetted droplets on the evaporation is investigated. In addition, the model to predict evaporation rate of the picoliter droplet on different substrates at a heated condition is developed using approximations for picoliter droplet.