The effect of fuel mixing on soot structure with methane, ethane, and propane to ethylene-base counterflow diffusion flames has been investigated by measuring the volume fraction, number density, and particle size of soot by adopting the light extinction/scattering techniques. The experimental result showed that the mixing of ethane and propane in ethylene diffusion flame increased soot volume fraction while the mixing of methane decreased. As compare to the ethylene-base flame, the diameters of soot particles for mixture flames are slightly smaller. While the soot number densities for the mixture flames are much higher. Thus, the increase in the soot volume fraction can be attributed to the appreciably increased soot number density by the fuel mixing.