Purpose: The purpose of this study was to identify the predictors of Quality of Life (QOL) among workers in public health corporations. Methods: Data were composed of 213 office workers in three public enterprise in Seoul. Data were collected from February 11 to March 20, 2007. Data were analyzed with descriptive statistics, t-test, ANOVA, Pearson correlation and stepwise multiple regression using SPSS Win 12.0 program. Results: The mean QOL of the study participants was 82.60. The QOLs of the workers were significantly different according to perceived health status of workers and working hours per week. The QOL of the workers was negatively related to working hours, job stress, and depression, and positively related to the health status, self-esteem, and health promotion lifestyle (HPLS). Significant predictors of QOL were HPLS ${eta}=.420$), depression (${eta}=-.291$), self-esteem (${eta}=.261$), and hours of work per week (${eta}=-.114$), which explained 63% in the variance of QOL. Conclusion: These results suggest that strategies to enhance HPLS could improve the level of QOL. Further investigations of the direct relationship between QOL and health promotion program is warranted.