Purpose: The aim of this study was to determine the effect of proprioceptive neuromuscular facilitation (PNF) leg patterns on muscle activation of paretic and nonparetic arm in post-stroke hemiparetic subjects. Methods: Eighteen hemiparetic patients participated in this study. Each subject was asked to perform four PNF leg patterns against maximal manual resistance on nonparetic leg. EMG data were collected from biceps and triceps on the paretic and nonparetic side. The measured EMG data was digitized and processed to root mean square (RMS) and expressed as percentage maximal voluntary isometric contraction (％MVIC). The data were analyzed using paired t-test and one-way analysis of variance (ANOVA) with repeated measures to determine the statistical significance. Results: The results of this study were summarized as follows: Firstly, during four PNF patterns application, all of the ％MVIC values of biceps and triceps in paretic and nonparetic arms increased significantly compared with resting condition (p<0.05). Secondly, there was a significant difference in ％MVIC of paretic biceps between PNF leg pattern 1 and 4 (p<0.05). Conclusion: In conclusion, regardless of the kinematic components of PNF leg patterns, all of the muscle activation of biceps and triceps in paretic and nonparetic arm was significantly increased. The finding of this study indicates that irradiation effect caused by PNF leg patterns is beneficial for increased muscle activation of both paretic and nonparetic arms in post-stroke patients.