In this study, fretting wear tests of nuclear fuel rods have been performed by using two kinds of spacer grid springs with a concave and a convex shape in room temperature dry and distilled water conditions. The objectives were to examine the variation of the wear mechanism with increasing fretting cycles and to evaluate the difference of the wear debris detachment behavior at each test environment. From the test results, the wear volume of each spring condition increased with increasing fretting cycles regardless of the test environments. However, the wear rate did not show a regular tendency and apparently changed with increasing fretting cycles. This is because the formation of the wear particle layer and/or the variation of the contact condition between the fuel rod and spring surfaces could affect a critical plastic deformation for detaching the wear debris. Based on the test results, the relationship between the wear behavior of each spring shape and test environment condition, and the variation of the surface characteristics are discussed in detail.