In this study, we investigated the existence of melamine and cyanuric acid in cigarette smoke because leaf tobacco contains lots of nitrogen compounds and they are capable of pyrolytic synthesis while smoking. The extraction and derivatization conditions of two compounds were performed according to the procedure of U.S. Food and Drug Administration(FDA). Ky3R4F as reference cigarette was smoked on ISO condition(Puff volumne : 35 ml, duration : 60 sec, Interval : 2 sec). Compared with the results Ky3R4F and standard solution, retention time of two compounds was not corresponded. As a result, we concluded that melamine and cyanuric acid were not existed in cigarette smoke.