Objectives : The purpose of this study was to investigate the effects of acupuncture on autonomic nervous system in anxiety patients and normal subjects by using SCR(Skin Conductance Response). Methods : The study group consists of 15 anxiety patients as the experimental group stimulated by verum acupuncture, 15 anxiety patients as the control group stimulated by sharm acupuncture, and 15 normal subjects stimulated by verum acupuncture. We measured GSR of experimental and control group, normal subjects during Working Memory Test and acupuncture stimulation. Results : 1. Measured value of SCR during Working Memory Test was significant different in control group, but it was not significant different in experimental group. 2. SCR value of three groups was significantly decreased during acupuncture stimulated. There was only significantly SCR value change during 0min.-5min, of first acupuncture. The section of the other groups was no significantly different. 3. STAI scores of experimental group and control group were significantly decreased. Conclusions : The study results suggest that acupuncture stimulation and sham acupuncture also effective on activity in the sympathetic nervous system and STAI scores.