A comparative experimental study of prestressed continuous steel-concrete composite beams was carried out. Two continuous composite beams were tested, one of which was plain continuous steel-concrete composite beam, while the other was a composite beam prestressed with external tendons. Cracking behavior and the load carrying capacity of the beams were investigated experimentally. Full plasticity was developed in the mid-span section each beam, the maximum moments attained at the internal support sections however were governed by local buckling which was related to the slenderness of composite section. It was found that in hogging moment regions, the ultimate resistance of an externally prestressed composite beam would be governed by either distortional lateral buckling or local buckling, or interactive mode of these two buckling patterns. The results show that exerting prestressing on a continuous composite beam with external tendons will increase the extent of internal force and moment redistribution in the beam. The influences of local and distortional buckling on the behaviors of the composite continuous beams are discussed. The Moment redistribution and the load carrying capacity of the prestressed continuous composite beams are evaluated, and it is found that at the ultimate state, the moment redistribution in the prestrssed continuous composite beams is greater than that in non-prestressed composite beams.