Three-dimensional numerical analyses of mechanical ventilation system in vent shaft of subway in operation are carried out in relation with the different air flow passage of vent shaft and two ventilation operation modes of push/pull, The ventilation characteristics of vent shaft with regard to the shape change are evaluated. And the air flow rate through the vent shaft by ventilation system is measured within subway in operation to assess the accuracy and applicability of the numerical analysis method. The decrease of air flow rate due to vent-shaft change are between 0.7 to 2.2% in the cases examined.