The instability wave formed near nozzle region grows to vortex with large scale in downstream region of spray. It plays an important role in the fuel-air mixing, combustion process and engine exhaust emissions in direct injection diesel engine. The objective of this study is to analyze effect of variant parameters (injection pressure, ambient gas density, etc.) and fuel properties on spray instability near nozzle region. Spray structure near nozzle region was investigated using a magnification photograph. A pulsed Nd-YAG laser was used as a light source, and image was taken by CCD camera. The following conclusions are drawn from this experimental analysis. In low ambient density, the effect of fuel properties on spray instability near nozzle region is dominant. In high ambient density, the effect of ambient gas on spray instability near nozzle region is dominant. High jet velocity has strong influence on spray instability.