This paper prescribed the structural integrity of the API 5L X65 pipeline subjected to tensile pre-strain. The effects of pre-strain on the mechanical properties of API 5L X65 pipe were substantially investigated through a variety of the experimental procedures. Axial tensile pre-strain of 1.5, 5 and 10% was applied to plate-type tensile specimens cut from the pipe body prior to mechanical testing. Tensile test revealed that yield strength and tensile strength were increased with increasing tensile pre-strain. The increasing rate of the yield strength owing to the pre-strain is greater than that of the tensile strength. However, the pre-strain up to 5% had a little effect on the decreasing of the fracture toughness. The structural integrity of the API 5L X65 pipeline subjected to large plastic deformation was evaluated through the fitness-for service code.