Purpose: The purpose of this study was to develop online health promotion an education program for university students and to evaluate its changes. Method: An online health promotion education program was established through analysis, designing, development, implementation and evaluation stages. This was a quasi-experimental, one group pretest-posttest design. The subjects of this study were 97 students in one university, and they were provided the online class for 16 weeks. Data was analyzed by descriptive statistics and paired t-test. Result: The results of program evaluation were positive, which included instruction function 3.94, system construction 4.14, system function 3.90, and overall evaluation 4.03 on the scale of 5. The posttest scores of health promoting lifestyle(t=-2.316, p=0.024) was significantly higher than those of the pretest. Conclusion: This study shows that the online health promotion education program was an effective educational method to improve health promotion lifestyle for the university students.