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Comparison of Spectral Data of Metabolites Collected from Bruker and Varian 600 MHz Spectrometers
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  • Comparison of Spectral Data of Metabolites Collected from Bruker and Varian 600 MHz Spectrometers
  • Comparison of Spectral Data of Metabolites Collected from Bruker and Varian 600 MHz Spectrometers
저자명
Kang. Woo-Young,Chae. Young-Kee
간행물명
Journal of the Korean magnetic resonance society
권/호정보
2009년|13권 1호|pp.7-14 (8 pages)
발행정보
한국자기공명학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The spectral data were collected from the two 600 MHz spectrometers from the two major manufacturers, Broker and Varian. The samples were prepared to create standard curves for quantitative measurements of metabolite concentrations. Instead of employing one-dimensional $^1H$ experiments, the two-dimensional $^1H-^{13}C$ HSQC experiments were performed for better separation of resonances. For some resonances, the high salt condition hindered the linear correlation between the intensity and actual metabolite concentration. Excluding overlapped ones, most resonances showed good linearity. Although the Varian spectrometer showed better linearity, both spectrometers were able to generate acceptable standard curves. From this data, we could identify resonances that could be used to better quantify the concentrations of the particular metabolites. With these standard curves, the quantitative measurements of the metabolites from the real samples will be facilitated.