The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials, However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsin-solubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (${alpha}1)_2{alpha}2$ heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7$^{circ}C$, which is about 12$^{circ}C$ lower than mammalian collagen. Immunoblotting assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.