This study was carried out to find out the of attitude and knowledge to blood donors in college and University students to provide basic data necessary for managing current blood donation system. The data were collected from 844 students from september 8 to 21, 2008 by using a self-administered questionnaire. The results were as follows: The major demographic characteristics were female 55.8% and the age group of 20-24s 60.5%, no religion 43.5%, blood type A 34.8% and so on. The major motivation for blood donation were to obtain a blood donation certificate to knowing necessary to blood donation 27.1%, take a voluntary service scored and souvenir 18.7%, and worthwhile and pride 9.9%. The experience the blood donation was 53.8%(male 69.2%, female 41.6%) of participants. Students who had more knowledge and desirable attitude about blood donation experienced more blood donations. It is important to clarify factors affecting blood donation practice and to encourage the public education and campaign which enable public has accurate knowledge and positive attitude about blood donation.