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양전하 및 음전하 필터를 이용한 해수 중 Norovirus Surrogate의 회수
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  • 양전하 및 음전하 필터를 이용한 해수 중 Norovirus Surrogate의 회수
저자명
이희정,오은경,유홍식,신순범,손명진,정진이,김영목,윤호동,Lee. Hee-Jung,Oh. Eun-Gyoung,Yu. Hong-Sik,Shin. Soon-Bum,Son. Myeong-Jin,Jung. Jin-Yi,Kim. Young
간행물명
한국수산과학회지
권/호정보
2009년|42권 3호|pp.238-242 (5 pages)
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한국수산과학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Recently coastal seawater has been contaminated by enteric viruses such as the norovirus via untreated groundwater globally. Accordingly, the consumption of molluscan shellfish from seawater that has been contaminated with fecal material has become an important issues. The levels of enteric viruses in seawater are low and recovery and concentration of the virus from large volumes of water is difficult. We compared the effectiveness of two representative method of concentrating virus using negatively and positively charged filters. The mean retention of seeded FCV by HAMF and NCCF was 48% and 78%, respectively. Overall, the recovery of NCCF was 43.3$pm$11% better than that of HAMF. However, the eluate obtained by using beef extract solution in the NCCF procedure caused an inhibitory effect on the RT-PCR; therefore, it was necessary to employ a PCR inhibitor removal procedure. The HAMF eluate contained no PCR inhibitors, but HAMF was not an effective method of concentrating the virus from large volumes of natural seawater due to clogging.