This paper proposes a position estimation of a mobile robot based on USN(Ubiquitous Sensor Network) and encoder, and development of tele-operation system using Internet. USN used in experiments is based on ZigBee protocol and has location estimation engine which uses RSSI signal to estimate distance between nodes. By distortion the estimated distance using RSSI is not correct, compensation method is needed. We obtained fuzzy model to calculate more accurate distance between nodes and use encoder which is built in robot to estimate accurate position of robot. Based on proposed position estimation method, tele-operation system was developed. We show by experiment that proposed method is more appropriate for estimation of position and remote navigation of mobile robot through Internet.