The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column ($250;mm;{ imes};4.6;mm$, $5;{mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230nm). The flow rate was 1.0 ml/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was $0.090;{pm};0.03%$ in P. lactiflora extract(control), $0.102;{pm};0.00%$ in P. lactiflora extract fermented with Paecilomyces japonica, $0.056;{pm};0.01%$ in P. lactiflora extract fermented with Ganoderma lucidum, $0.093;{pm};0.00%$ in P. lactiflora extract fermented with honey and $0.046;{pm};0.00%$ in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was $4.506;{pm};0.13%$ in control, $2.599;{pm};0.04%$ in P. lactiflora extract fermented with Paecilomyces japonica, $1.222;{pm};0.03%$ in P. lactiflora extract fermented with Ganoderma lucidum, $2.750;{pm};0.05%$ in P. lactiflora extract fermented with honey and $0.847;{pm};0.00%$ in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.