Objectives: Examine the difference of Chosun acupuncture from the acupuncture in Myung, Chung Dynasty Method: This paper examines the acupuncture mentioned in the classic of Introduction to Medicine For this, the special traits of Introduction to Medicine acupuncture was analyzed. Secondly, the research on the contents of Introduction to Medicine acupuncture quoted in the classic of Essential Rhymes of Acupuncture and Moxibustion, Treasured Mirror of Eastern Medicine, and Experimental Prescriptions of Acupuncture and Moxibustion was carried out. Result: Introduction to Medicine put much importance on "method of acupuncture following the five circuits and six qi" (子午流注針法) and Song of Acupuncture and Moxibustion through comparing "eight methods of acupuncture following the five circuits and six qi" (子午八法) and "scattered needling method". The doctors in Chosun Dynasty, howevery, did not adopt this theory with their own standards on acupuncture. On the contrary, they put more importance on the inquiry of historical evidence on river point and extraordinary points. Conclusion: The acupuncture studies of Chosun Dynasty had already formed its own standards on the medical theories, and its direction to which it was headed was different from that of the Myung, and Chung Dynasty in China.