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Study on In Vitro Development of Vitrified-Thawed Porcine Oocytes
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  • Study on In Vitro Development of Vitrified-Thawed Porcine Oocytes
  • Study on In Vitro Development of Vitrified-Thawed Porcine Oocytes
저자명
Chung. Gun-Ho,Rhee. Man-Hee,Kim. Sang-Keun
간행물명
韓國受精卵移植學會誌
권/호정보
2009년|24권 2호|pp.115-119 (5 pages)
발행정보
한국수정란이식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrifiedthawed porcine oocytes were examined. Oocytes were cultured in TCM-199 medium supplemented with 5% FBS at $38^{circ}$C in 5% $C0_2$ and air. The percentage of monospermy in the toxicity group and vitrification group (22.0 ${pm}$ 3.0% and 31.5 ${pm}$ 3.5%) was decreased compared with that of the control group (44.0 ${pm}$ 4.0%). The percentage of in vitro development to blastocyst in the toxicity group and vitrification group (12.0 ${pm}$ 2.5% and 14.8 ${pm}$ 2.8%) was decreased compared with that of the control group (28.0 ${pm}$ 3.0%, p<0.05). The survival and in vitro developmental rate of oocytes vitrification-thawed with EDS and EDT + TCM-199 medium supplemented with 0.1% PVA were 46.3 ${pm}$ 3.0%, 54.5 ${pm}$ 3.8% and 14.8 ${pm}$ 2.5%, 16.4 ${pm}$ 2.7%, respectively. This results were lower than the control group (28.0 ${pm}$ 3.5%). The in vitro developmental rate of embryos vitrified with EDS and EDT supplemented PVA did not have a significant difference. The survival and in vitro developmental rate of vitrified-thawed morula and blastocyst embryos were 44.2 ${pm}$ 3.5%, 17.3 ${pm}$ 3.0% and 48.1 ${pm}$ 4.2%, 18.5 ${pm}$ 3.5%, respectively. Vitrified morulae and blastcyst embryos had a lower survival and developmental rates than their control counterparts.