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Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method
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  • Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method
  • Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method
저자명
Hu. Jiang-Ning,Zhu. Xue-Mei,Adhikari. Prakash,Li. Dan,Kim. In-Hwan,Lee. Ki-Teak
간행물명
Journal of food science and nutrition
권/호정보
2009년|14권 3호|pp.260-264 (5 pages)
발행정보
한국식품영양과학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression ($r^2$>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94$sim$4.27 and 1.77$sim$ 4.88%, respectively. The tocopherol recoveries ranged from 91.44$sim$108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of $alpha$, $gamma$, and $delta$-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.