Objective: We performed this study to check relationship of Cold-Heat attribute analyzed quantitatively by questionnaire with acoustic analysis index. Method : We checked a questionnaire composed of 15 items about the contents of Cold-Heat and asked 83 subjects to answer in the form Likert-like 7-points score. And then, we extracted Cold-Heat attribute from heat score, cold score, heat index and cold index. we measured the acoustic analysis indexes of cardinal vowels by Dr. speech program. Afterward, the data were analyzed by correlation analysis. Results : All cardinal vowels is positive correlated with cold score, heat score and cold index. NNE of vowel /a/ is negative correlated with cold index. Shimmer and F0 tremor of vowel /e/ is negative correlated with cold index. Jitter of vower /u/ is positive correlated with Cold score.