In general, manual calibration is commonly used for the stream water quality modelling. Because the manual calibration depends upon the subjectivity and experience of the researcher, it has a problem with the objectivity of the modelling. Thus, the interest about the automatic calibration by the optimization technique is deeply increased. In this study, Influence coefficient algorithm and Genetic algorithm are introduced to develop an automatic calibration model for the QUAL2K that are the latest version of the QUAL2E. Genetic algorithm, used in this study, is very simple and easy to understand but also applicable to any complicated mathematical problem, and it can find out the global optimum solution effectively. The developed automatic calibration model is applied to the Gangneung Namdaecheon. The calibration results about the 11 water quality variables show the good correspondence between the calculated and observed water quality values.