This study examined the difference for the ability of self-directed learning and information literacy(the ability to use information) between small group-cooperative instruction of ICT practical environment and explanatory instruction using ICT of general classroom in middle school science education. The 138 students(7th grade) participated in this study were classified in two group, experimental group(68) and controlled group(70). Experimental small group consisted of 5-6 students who had different levels and the ability of each the group was equal. The teaching of experimental group was made in the science lab which was equipped with computers to connect super high-speed Internet. The teaching of control group was made in general classroom. Under these two environment, this study was executed during 10 weeks. As the result, Small group-cooperative learning using ICT was more effective in diminishing the gap of information literacy in middle school students. And in the ability of self-directed learning, the small group learning using ICT was also more effective than general classroom instruction.