This experimental study sought to investigate the characteristics of the exhaust emissions, and nanoparticle size distribution of particulate matter (PM) emitted from diesel engines fueled with 20% biodiesel-diesel blended fuel (BD20). The study also investigated the conversion efficiency of the warm-up catalytic converter (WCC). The emission characteristics of HC, CO, NOx and nano-sized PM were also observed according to engine operating conditions with and without exhaust gas recirculation (EGR). The study revealed that the maximum torque achievable with the biodiesel-diesel blended fuel was slightly lower than that achievable with neat diesel fuel at high-load conditions. Smoke was decreased by more than 20% in all 13 modes. While the CO and THC emissions of BD20 slightly decreased, the NOx emission of BD20 increased by 3.7%. Measured using the scanning mobility particle sizer (SMPS), the total number and total mass of the nanoparticles in the size range between 10.6nm and 385nm were reduced by about 10% and 25%, respectively, when going from D100 to BD20. The particle number and mass for both fuels were reduced by about 43% when going from with EGR to without EGR. When EGR was applied in the engine system, the particle number and mass were reduced by 24%, and 16%, respectively, when going from D100 to BD20.