The fashion industry saw the emergence of environment-friendly and natural fabric materials recently. The naturalism trend has established sectors in the textile and fashion industry also. Ramie and hemp have long been favored fabric materials in Korea especially for summer. The hand-related mechanical/physical properties of the fabric specimens were analyzed using a fabric objective measurement system, KES(Kawabata Evaluation System). Subjective evaluation was also implemented based on a series of selected adjectives. Studies on natural dyeing methods are important since the establishment of scientific approach is needed in terms of environment and human safety along with the reproduction of traditional natural dyeing methods. In this study, linen, ramie, hemp, and cotton/polyester/linen union fabric specimens were used for the natural dyeing using Rubia akane Nakai with the mordant of gallnut. ${Delta}E$ values of dyed fabric specimens, mordanted and dyed once using Rubia akane Nakai, increased significantly compared to those of repeatedly dyed fabric specimens without mordanting.