This study analyzed and examined the anti-oxidant properties of Saengmaegsan chocolate, and blood glucose levels were examined according to the Saengmaegsan chocolate intake. The total carbohydrate content of the chocolate was 57.8%, crude fat was 31.5%, crude ash was 1.6%, and crude protein was 4.0%. In Saengmaegsan chocolate. Furthermore, total polyphenol content of Saengmaegsan chocolate was 191.52 mg/100 g and DPPH scavenging activity was 87.06%. The radical scavenging activity of the Saengmaegsan chocolate was higher than that of normal milk chocolate. Also, blood glucose levels were significantly lower, by Saengmaegsan chocolate intake, than by normal milk chocolate intake. These results indicate that Saengmaegsan chocolate can be used as a safe and is clinically applicable as a supplementation of in diet therapy for both healthy and diabetic persons.