The purpose of this study is to examine the effect of the functional shoes through the kinetic comparison of normal-design running shoes and spring-loaded running shoes. For this, 12 healthy females from the age from 30 to 40 years participated in the EMG and ground reaction force experiment with testing kinetic variables. 12 subjects walked at the velocity of 1.7m/s. After analyzing variables in the spring-loaded running shoes and normal-design running shoes, the following conclusions were obtained; For the ground reaction force, spring-loaded running shoes have larger antero-posterior GRF than normal-design running shoes in the first and second apexes of antero-posterior ground reaction force. For the analysis of EMG, spring-loaded running shoes showed the higher muscle activation of rectus femoris muscle than norma-design running shoes. So the spring-loaded running shoes help improvement muscle strength of knee extensor.